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. 2006 Feb 9;34(3):976–985. doi: 10.1093/nar/gkj501

Figure 4.

Figure 4

Representative autoradiographs at nucleotide resolution showing the repair of CPDs in the TS and NTS of the MspI fragment of MET16 in wild type, gcn5Δ and ada2Δ cells grown in 1 mM (repressing) and 10 µM methionine conditions (derepressing). Sanger A+G and T+C sequencing ladders are included to determine the position of the CPDs induced. U, untreated cells; 0, cells treated with UV light no repair; (1–4), UV-treated cells allowed to repair the damage for 1–4 h. The intense top band corresponds to the undamaged MspI fragment of MET16, the bands below represent DNA fragments with a CPD lesion that was cut by the CPD-specific endonuclease. Cbf1p-binding site (closed box), Gcn4p-binding site (stripped box), TATA-box (open box) and coding region (grey box). Note: differences in band migration at the bottom of the autoradiographs are due to the fact that ada2Δ samples were run in different gels to PSY and gcn5Δ.