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Masses of virion-derived HIV-1 Env fractions
| Env fraction | Mass [kDa (no. of subunitsa)] measured by:
|
|
|---|---|---|
| Sedimentation equilibrium (wt avg) | STEM (no. avg) | |
| 48 | 488.5 (3.32) | 457 (3.10) |
| 49 | 525.7 (3.57) | |
| 50 | 459.9 (3.12) | 448 (3.04) |
| 51 | 471.9 (3.20) | |
The mass of one subunit (147.3 kDa) was estimated by summing the protein mass deduced from the amino acid sequence and a value of 1.739 kDa for each potential N-linked glycosylation site (see the text regarding sedimentation equilibrium analysis).
