FIG. 1.
The tvbr allele is expressed as a normal-sized mRNA transcript. Northern blot analysis was performed with mRNA samples taken from CEFs with the indicated tvb genotypes. The nylon membrane was probed with a 32P-labeled tvb-specific probe derived from the BK-9 tvb genomic DNA clone as described previously (9). Autoradiography was performed at −80°C with intensifying screens.