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Generation of two overlapping cDNA fragments derived from tvbr mRNA. A schematic drawing of the tvb cDNA clone (GenBank accession number AR071759) is shown along with the location of two overlapping tvbr cDNA fragments generated during this study. tvbr cDNA fragment 2 contains a unique 5′ sequence derived by rapid amplification of cDNA ends which extends 130 bp beyond that reported previously. The point mutation is in the region of overlap and is indicated by an asterisk.
