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A 2.1-kb BfaI restriction fragment is diagnostic of the tvbr allele. BfaI-digested genomic DNA samples prepared from CEFs with the tvb genotypes indicated were subjected to Southern blot analysis with a 32P-labeled tvb-specific DNA probe under standard conditions. Autoradiography was performed for 10 days at −80°C with intensifying screens. The position of the 2.1-kb DNA fragment that is unique to tvbr is indicated with an arrow.
