Table 3.
Peripheral blood lymphocyte subpopulation immunophenotyping and age-matched EuroFlow reference ranges.
| Estimated cellularity in relation to the total number of nucleated cells examined | ||
|---|---|---|
| Population | Events/ul | Range/ul |
| Linphocytes | 2150 | 2500-9920 |
| B- cells | 88 | 485-3950 |
| Pre-Germinal Centre | 70 | 455-3585 |
| Post-Germinal right (post-GC CBM) | 17 | 8-405 |
| BCM ‘‘Unswitched¨/Plasmatic cells (CP) | 13 | 3-340 |
| BCM ¨Switched¨/CP | 4 | 4-100 |
| T-cells | 1924 | 1715-7500 |
| CD4+CD8- | 1013 | 1275-5460 |
| Naive CD4+ | 652 | 1100-5170 |
| CD4+Central/Transitional Memory | 279 | 150-835 |
| CD4+ Effector Memory | 80 | 0.2-40 |
| Effector TD CD4+ | 2 | 0-7 |
| CD8+ CD4- | 714 | 370-2000 |
| Naive CD8+ | 373 | 340-1805 |
| CD8+ central/transitional memory | 84 | 25-475 |
| Effector memory CD8+ | 87 | 0-135 |
| CD8 + TD Effectors CD27+d | 26 | 0-365 |
| CD8+ TD Effectors CD27- | 145 | 0-220 |
| CD4-CD8-/dim TCRgd+ | 38 | 45-430 |
| CD4-CD8-/dim TCRgd- | 157 | 10-70 |
| NK cells | 138 | 180-1330 |
| Eosinophils | 82 | 50-695 |
| Neutrophils | 417 | 775-8800 |
| Monocytes | 254 | 390-2950 |
| Monocytes CD16+ | 70 | 5-75 |
| Plasmocytoid dendritic cells | 3 | 9-60 |
| Basophils | 18 | 5-75 |
Patient values are expressed as absolute counts (events/µL). Reference ranges correspond to the 5th–95th percentiles of the healthy reference cohort aged 1–11 months old (n= 23), established using the standardized EuroFlow Primary Immunodeficiency Orientation Tube protocol.21.