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Confocal images of individual live nonpermeabilized (A) and permeabilized (B) cells from immunofluorescence studies. MDTF cells expressing receptors bearing C-terminal HA epitope tags were incubated with monoclonal antibody HA.11 and then stained with a fluorescein-conjugated second antibody. 1, MDTF-Pit1, untagged negative control; 2, MDTF-Pit1; 3, MDTF-Pit1-2A; 4, MDTF-Pit1-2B; 5, MDTF-Pit1-D550T; 6, MDTF-Pit1-2B-D550T.
