TABLE 1.
Infection properties of HA-tagged receptors
| Receptora | Titer (bfu/ml)b of:
|
|
|---|---|---|
| GALV | FeLV-B | |
| None | <10 | <10 |
| Pit1 | 4.0 × 106 | 1.0 × 106 |
| Pit1-HA | 5.2 × 106 | 1.7 × 106 |
| Pit1-2A-HA | <10 | 7.6 × 102 |
| Pit1-D550T-HA | 1.8 × 106 | 0.3 × 106 |
| Pit1-D550K-HA | 0.7 × 106 | 2.7 × 104 |
| Pit1-2B-HA | <10 | <10 |
| Pit1-2B-D550T-HA | 2.4 × 106 | 0.3 × 106 |
a
Receptors are stably expressed in MDTF cells transduced by VSV G-enveloped vectors expressing receptor cDNA genomes, as described in Materials and Methods.
b
Titers are expressed as the mean numbers of blue foci (bfu) on target MDTF cells or MDTF cells expressing various receptors per milliliter from three experiments.