Table 2.
Primers for constructing truncated variants.
| Name | Sequences (5′-3′) |
|---|---|
| GFP-B169L-F | AATTCTGCAGTCGACGGTACCGCCACCATGAATGTGGACTTCATCGCCG |
| GFP-B169L-R | TTATCTAGATCCGGTGGATCCTTAGTTGTTATACTGAGAAGGCATGATCAC |
| P1-F | AATTCTGCAGTCGACGGTACCATGAATGTGGACTTCATCGCCG |
| P1-R | TTATCTAGATCCGGTGGATCCTTAGTTGCTCTTGTTGTAGATGTCGTTGAAGGGCTCGCAGGTGTAGATC |
| P2-F | AATTCTGCAGTCGACGGTACCATGAAGATCTACACCTGCGAGCCCTTCAACGACATCTACAACAAGAGCAAC |
| P2-R | TTATCTAGATCCGGTGGATCCTTAGATGATCTCATCGCCTCCCTT |
| P3-F | AATTCTGCAGTCGACGGTACCATGCACGATAAGTACAAGGGAGGCG |
| P3-R | TTATCTAGATCCGGTGGATCCTTAGATCTTCTTGGGCTGATCCTCC |
| P4-F | AATTCTGCAGTCGACGGTACCATGAACGAGCTGGAGGAGGATCAG |
| P4-R | TTATCTAGATCCGGTGGATCCTTACAGGGGCTTGGAGTCGGG |
| P5-F | AATTCTGCAGTCGACGGTACCATGAAGCCCGTGAGCCTGCCC |
| P5-R | TTATCTAGATCCGGTGGATCCTCAGTTGTTATACTGAGAAGGCATGA |
| P5-1-F | GAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCATGG |
| P5-1-R | TTATCTAGATCCGGTGGATCCCACTTCCTGCAGAGGCACCAGGGGCTTGGAGTCGGGCAGGCTCACGGGCTTCGGGCCCGCGGTACCGTC |
| P5-2-F | GAACCGTCAGATCCGCTAGCGCTACCGGTCGCCACCATGG |
| P5-2-R | TTATCTAGATCCGGTGGATCCTCAGTTGTTATACTGAGAAGGCATGATCACTTCCTGCAGAGGCACCAGGGGCGGGCCCGCGGTACCGTC |
| P5-1-1-F | CCGCCCGTGAGCCTGCCCGACTCCAAGCCCCTGGT |
| P5-1-1-R | TCGGGCAGGCTCACGGGCGGGCCCGCGGTACCGTC |
| P5-1-2-F | CCCGGTGAGCCTGCCCGACTCCAAGCCCCTGGTGC |
| P5-1-2-R | AGTCGGGCAGGCTCACCGGGCCCGCGGTACCGTC |
| P5-1-3-F | CCGAGCCTGCCCGACTCCAAGCCCCTGGTGCCTCT |
| P5-1-3-R | TTGGAGTCGGGCAGGCTCGGGCCCGCGGTACCGTC |
| P5-1-4-F | CCCGCTGCCCGACTCCAAGCCCCTGGTGCCTCT |
| P5-1-4-R | TTGGAGTCGGGCAGCGGGCCCGCGGTACCGTCG |
| P5-1-5-F | CCGCCCGACTCCAAGCCCCTGGTGCCTCTGCAGGA |
| P5-1-5-R | AGGGGCTTGGAGTCGGGCGGGCCCGCGGTACCGTC |
| P5-1-6-F | CCGGACTCCAAGCCCCTGGTGCCTCTGCAGGAAGT |
| P5-1-6-R | ACCAGGGGCTTGGAGTCCGGGCCCGCGGTACCGTC |
| P5-1-7-F | CCGTCCAAGCCCCTGGTGCCTCTGCAGGAAGTGGGA |
| P5-1-7-R | AGGCACCAGGGGCTTGGACGGGCCCGCGGTACCGTC |
| P5-1-8-F | CCGAAGCCCCTGGTGCCTCTGCAGGAAGTGGGATC |
| P5-1-8-R | AGAGGCACCAGGGGCTTCGGGCCCGCGGTACCGTC |
| P5-1-9-F | CCGCCCCTGGTGCCTCTGCAGGAAGTGGGATCCAC |
| P5-1-9-R | TGCAGAGGCACCAGGGGCGGGCCCGCGGTACCGTC |
| P5-1-10-F | GGAAGGATCCACCGGATCTAGATAACTGATCATAA |
| P5-1-10-R | TAGATCCGGTGGATCCTTCCTGCAGAGGCACCAGG |
| P5-1-11-F | GCAGGGATCCACCGGATCTAGATAACTGATCATAA |
| P5-1-11-R | TAGATCCGGTGGATCCCTGCAGAGGCACCAGGGG |
| P5-1-12-F | TCTGGGATCCACCGGATCTAGATAACTGATCATAA |
| P5-1-12-R | TAGATCCGGTGGATCCCAGAGGCACCAGGGGCTT |
| P5-1-13-F | GCCTGGATCCACCGGATCTAGATAACTGATCATAA |
| P5-1-13-R | TAGATCCGGTGGATCCAGGCACCAGGGGCTTGGA |
| P5-1-14-F | GGTGGGATCCACCGGATCTAGATAACTGATCATAA |
| P5-1-14-R | TAGATCCGGTGGATCCCACCAGGGGCTTGGAGTCG |
| P5-1-15-F | CCTGGGATCCACCGGATCTAGATAACTGATCATAA |
| P5-1-15-R | TAGATCCGGTGGATCCCAGGGGCTTGGAGTCGGG |
| P5-1-16-F | GCCCGGATCCACCGGATCTAGATAACTGATCATAATC |
| P5-1-16-R | TCTAGATCCGGTGGATCCGGGCTTGGAGTCGGGCAG |
| P5-1-17-F | CAAGGGATCCACCGGATCTAGATAACTGATCATA |
| P5-1-17-R | AGATCCGGTGGATCCCTTGGAGTCGGGCAGGCT |
| P5-1-18-F | CTCCGGATCCACCGGATCTAGATAACTGATCATAA |
| P5-1-18-R | TCTAGATCCGGTGGATCCGGAGTCGGGCAGGCTCAC |