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Dependency on DNA polymerase activity for HHV-6B gene expression. Molt-3 T cells infected by HHV-6B were incubated in the presence or absence of the DNA polymerase inhibitors PAA or APH for 24 h. RNA was isolated, and real-time PCR was performed on cDNA. The percentage of PAA- and APH-mediated inhibition of viral gene expression was calculated on the basis of the real-time PCR crossing points as described in Materials and Methods.
