TABLE 1.

Primer pairs used for analysis of HHV-6B gene expression and sequencing

Name	Primer coordinatesa	Nucleotide sequenceb (5′-3′)		Size of amplified product (bp)
		Forward	Reverse
β2mc		CAA GCA GAG AAT GGA AAG TC	GAT GCT GCT TAC ATG TCT CG	269
B3	7351-7485	GTC TAT CCG CCT GCG TGT AT	AGT AAC ACG GCA CGT AAG CA	135
B4	8972-9231	GCG AGA GAG AAA GAG AGG CA	GGG TCG GGG GTC TAT AAA AA	260
B5	9555-9733	AGA CAA CCA CGA AAA CGA CC	ACA TGG TAG AGC GGG AAA TG	179
U2	10519-10712	GAG ACG AAG TCC CTC ACG TC	TTT TGT CGC TCT CCG TTT CT	194
U7	15666-15956	ACG ACA AAC CTG CTG GTA GC	GGA TGT TGA ATG GGG AGT TG	291/200d
U8	17687-17882	TCC GTC TCT AGA TCG TGC AA	GGA ACT GCT GGC TGA GAT TC	196
U9	18225-18426	ATT TCC TGC GAA TGT TGG AC	ATA CAG AAG CCG GAA CGA TG	202
U11	21476-21671	ATC GAC GCT ATC CGA CTC TG	TGG GCC TCA CTC TTT AAT GG	196
U12	22652-22915	AGC TGT CCA AAC TCC TGC AC	GCG TTA TGT TTT GCG ACT CTG	187e
U14	25813-26010	TCA CAG GAA GAC AGC AAT CG	GGG AGC ACG TCT TCT TTG TC	198
U21	34591-34800	CCA TTT TAC CGC TTC TCC AA	TGG AGT TAG TGG GCG CTA GT	210
U22	34875-35085	ATC CAA AGC AAA CCA GCA AG	TCA ATC GGT GAT GGA GTC AA	211
U23	35607-35855	GAA TGC CCC GAC AAA ACT AA	GAG GCG CAG GAT ATT GAG AC	249
U27	38971-39167	GAA TCG TCG CTC TCG CTA TC	GAG GAA TCT CGC TTT GAA CG	197
U29	42950-43155	CGT AAG CCC GTG AAT GTT TT	GCG TGA GAA CCG CTT TAA CT	206
U30	44406-44602	CCT CAA GAA CGG GAG AAT GA	GCC ATG TGG TTT GAG AGG AT	197
U35	55133-55324	CCA TTT TCT GAG CCA TGA CC	AAG TCT GTG AGC AGG GAA GG	192
U36	55854-56109	TCA CAT CCA AAG CGA AAA CA	GCG GAT AGT TAG GTC CGT GA	256
U37	57056-57255	GCA ATC ACC ATC AAC ACA CC	GCG TAT CAG CTC CAT GTC CT	200
U38	58157-58356	TGT GTT TGC GTC ATG TGA GA	TGT CCG CTC ATT TTG ATT TG	200
U39	62061-62501	AAG AGG CTG CCA AAT CAA AA	CAT TTT GCA GAG CCG TTA GA	441
U41	65450-65645	GTC ATA GAC CGG AGC ATC GT	TGA GGT GAT GAG GGA TAG GG	196
U47	78067-78261	CGG TCG AGG AAA ATC TGA AC	TCG ACG TCA GCG TCT ATC AC	195
U51	83839-84034	CAG CCA CTG CGG AGT TTT AT	GGC AAA GTC CAA TGT CCA CT	196
U54	87563-87761	CGA CCG TGG TTA GAC TTG GT	CGC ATC AAT TAC TCG CAA GA	199
U58	96103-96310	TAA GGC GGT TCC TGC AAT AC	GTC CGG CAA ACT GTA ATC GT	208
U69	106289-106489	CCT GGA TTT CGA CCA CTT GT	CGC AAA CAA CCT TTT CTC GT	201
U73	111144-111349	TGG GAA AGT TCA GAG CGA CT	AAA ACT CTT CGG CAG CAC AT	206
B6	119207-119308	GGG AAA CCT CAA AAA TGC AC	CTT AGG ACA CCA TCC AAG CC	102
B7	120962-121063	CCT AAT ACT TCT GAC GGG CG	TCA TCC AAA CCG AAA ACC TT	102
U81	123527-123730	AAT TCG GGA TGC TTC TTT CC	ATC TAA CCG TCC ACC CCT CT	204
U86	128815-129014	AAA GGA CTG GAG TCG AGC TG	CCA ACA TAC CTT CCC CTC AA	200
B8	142340-142636	TTA GGG TCC TCA CCC ACT TG	CCG CAG CTT CTG TTT TTC AT	297
U94	144100-144302	CGC CAC CAT TTT TCT TTG TT	TGC AAA GTG GTA CGC TCA AG	203
U95	147023-147222	CCA AGA AAG GCG AAA AGT TG	AAT GTC GGC AAG CAA TCT CT	200

^aCoordinates refer to the HHV-6B genome sequence in strain Z29 (accession no. AF157706).

^bThe annealing temperature was 62°C for all primer pairs used.

^cβ₂m, β₂-microglobulin.

^dUnspliced/spliced.

^eCovers a splice site.