TABLE 6.
Characteristics of mutant viruses
| Mutant virus | Repression in pustule formation | Infectivitya | Genome organizationb
|
|
|---|---|---|---|---|
| cDNA | ClampR | |||
| Δp40a | No | 3/3 (P, P, P) | Dicistronic | Monocistronic |
| Δp40b | No | 3/3 (S, S, S) | Monocistronic | Monocistronic |
| Δp29 | Yes | 3/3 (S, S, S) | Dicistronic | Dicistronic |
| p69aΔ25-253 | Yes | 3/3 (S, S, S) | Dicistronic | Dicistronic |
| p69aΔ25-271 | Yes | 3/3 (S, S, S) | Dicistronic | Dicistronic |
| p69aΔ25-287 | Yes | 3/3 (S, S, S) | Dicistronic | Dicistronic |
| p69aΔ25-299 | Noc | 3/3 (S, S, S) | Dicistronic | Dicistronic |
| p69aΔ25-312 | No | 1/3 (P) | Dicistronic | Monocistronic |
| p69aΔ25-325 | No | 1/3 (P) | Dicistronic | Monocistronic |
| p69aΔ25-360 | No | 3/3 (S, S, S) | Dicistronic | Mono/dicistronicd |
| p69aΔ25-384 | No | 3/3 (S, S, P) | Dicistronic | Mono/dicistronic |
| p69aΔ25-545 | No | 1/3 (P) | Dicistronic | Monocistronic |
| Δp69a | No | 3/3 (S, S, S) | Dicistronic | Dicistronic |
| Δp69b | No | 3/3 (S, S, S) | Monocistronic | Monocistronic |
a
Infectivity is shown as the ratio of infected plates (colonies) to total plates (colonies) regenerated from transfected spheroplasts and by the letters P and S, indicating partial and systemic infection.
b
Genome organization of mutant viruses is based on sequences of cDNA originally designed for in vitro transcription and sequences of ClampR fragments that were amplified on dsRNA recovered from transfected fungal colonies.
c
Although only the center of an infected colony produced stromal pustules, the surrounding area was suppressed in pustule production.
d
Both monocistronic and dicistronic genomes were detected in the progeny virus population.