FIG. 2.

Recombinant expression. All recombinant Gag and protease constructs are depicted. The dotted lines are the boundary of the coding region of the PERV protease. The construct numbers reflect the primer number. Primer sequences were #36 (GCGCGGATCCGAAGATAAAGATCAGGGGAGACG), #37 (CGCGAAGCTTTTAAAAAGAAATTTGAGCTCCCATC), #72 (GCGGTACCATG GAAGAAGATAAAGATCAGGGGAG), #C74 (CGCGAAGCTTAGTCATCTAATTGGAGGGTCAACAC), #74 (CGCGAAGCTTTTAGGGAGAATATAGTCGATATTCATC), #75 (CGCGAAGC TTTTACAACACAGTGATGGGTTTG), #90 (CGCGGATCCGGACCGAAGGTCCTAGCTCTAGAAGAAGATAAAGATCAGGGGAGAGGGGTTC), #C90 (CGCGGATCCGGACCAAGGATCCTAGCTCTAGAAGAAGATAAAGATCAG), #92 (GCGGT ACCATGGGACAGACAGTGACGACC), #93 (GCGGTACCTTAATCTTTATCT TCTTCTAGAGCTAGGAC), and #99 (CGCGGATCCTTGGTCCGGGTCCTAGCTCTAGAAGAAGATAAAGAT). LTR, long terminal repeat.