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. 2002 Aug;76(15):7430–7443. doi: 10.1128/JVI.76.15.7430-7443.2002

TABLE 2.

Quantitation of cell-associated inoculuma

Cell line Amt of cell-associated virus (cpm)
Ratio (amt with CHT/amt without CHT)
Without CHT With CHT
L929 37,864 24,800 0.65
A549 8,321 6,659 0.88
Vero 5,207 4,864 0.93
MEF 7,879 6,706 0.85
a

Equal amounts of purified [35S]methionine-labeled virus (strain Lang) were added to cells at 4°C. After adsorption, unbound virus was removed by a wash with ice-cold PBS. Medium was added (with or without CHT at 10 μg/ml). Cells were collected immediately (t = 0 h); cell-associated counts were recovered by acetone precipitation. Equivalent amounts of the cell extracts from samples with and without CHT were analyzed by SDS-PAGE. Cell-associated radioactivity in the viral λ proteins was quantitated after phosphorimaging using ImageQuant software (Molecular Dynamics).