TABLE 2.

Quantitation of cell-associated inoculum^a

Cell line	Amt of cell-associated virus (cpm)		Ratio (amt with CHT/amt without CHT)
	Without CHT	With CHT
L929	37,864	24,800	0.65
A549	8,321	6,659	0.88
Vero	5,207	4,864	0.93
MEF	7,879	6,706	0.85

^aEqual amounts of purified [³⁵S]methionine-labeled virus (strain Lang) were added to cells at 4°C. After adsorption, unbound virus was removed by a wash with ice-cold PBS. Medium was added (with or without CHT at 10 μg/ml). Cells were collected immediately (t = 0 h); cell-associated counts were recovered by acetone precipitation. Equivalent amounts of the cell extracts from samples with and without CHT were analyzed by SDS-PAGE. Cell-associated radioactivity in the viral λ proteins was quantitated after phosphorimaging using ImageQuant software (Molecular Dynamics).