TABLE 2.
Quantitation of cell-associated inoculuma
Cell line | Amt of cell-associated virus (cpm)
|
Ratio (amt with CHT/amt without CHT) | |
---|---|---|---|
Without CHT | With CHT | ||
L929 | 37,864 | 24,800 | 0.65 |
A549 | 8,321 | 6,659 | 0.88 |
Vero | 5,207 | 4,864 | 0.93 |
MEF | 7,879 | 6,706 | 0.85 |
Equal amounts of purified [35S]methionine-labeled virus (strain Lang) were added to cells at 4°C. After adsorption, unbound virus was removed by a wash with ice-cold PBS. Medium was added (with or without CHT at 10 μg/ml). Cells were collected immediately (t = 0 h); cell-associated counts were recovered by acetone precipitation. Equivalent amounts of the cell extracts from samples with and without CHT were analyzed by SDS-PAGE. Cell-associated radioactivity in the viral λ proteins was quantitated after phosphorimaging using ImageQuant software (Molecular Dynamics).