TABLE 3.

Effects of CHT on the replication of reovirus strain Lang in a panel of primary cell cultures and continuous cell lines^a

Cell type	Description	Viral yield (log10) at 24 h		Log10 increase in viral growth with CHT
		Without CHT	With CHT
L929	Mouse fibroblast cell line	1.70 ± 0.08	2.77 ± 0.16	1.07
A549	Human respiratory epithelial cell line	1.59 ± 0.09	2.54 ± 0.04	0.95
U937	Human monocyte cell line	1.19 ± 0.03	2.76 ± 0.06	1.58
BHK	Baby hamster kidney cell line	0.67 ± 0.06	1.07 ± 0.19	0.40
PrEp	Primary human prostatic epithelial cells	0.51 ± 0.03	1.61 ± 0.09	1.10
Vero	African green monkey kidney cell line	0.39 ± 0.33	2.64 ± 0.16	2.25
Primary tonsil	Primary human tonsillar cells	0.21 ± 0.13	1.25 ± 0.07	1.04
CRL-1492	Rat pancreatic tumor cell line	0.16 ± 0.08	2.47 ± 0.04	2.30
293	Human kidney epithelial cell line	0.13 ± 0.07	2.17 ± 0.11	2.26
MEF	Primary mouse embryo fibroblast cells	0.03 ± 0.09	1.67 ± 0.04	1.63
PrSc	Primary human prostate stromal cells	0.11 ± 0.05	0.57 ± 0.11	0.46
HL-60	Human promyelocytic leukemia cell line	−0.03 ± 0.08	1.21 ± 0.14	1.07
RAW	Mouse macrophage cell line	−0.04 ± 0.19	0.82 ± 0.25	0.86
3T6	Mouse embryo fibroblast cell line	−0.09 ± 0.08	0.27 ± 0.04	0.35

^aCells were infected at an MOI of 3 with reovirus strain Lang. After adsorption, cells were incubated at 37°C in media that either did or did not contain CHT at 10 μg/ml. Samples were harvested at 24 h p.i., and yields were determined by a modified CHT plaque assay on L929 cells. Each value represents the yield from three independent samples, calculated as described in Materials and Methods. Cells are ranked relative to the amount of viral growth in the absence of CHT; the least permissive cells are found at the bottom of the table.