Figure 1. An F2A sequence allows correct localization of nuclear and secreted protein reporters regardless of configuration.

Dual reporter strains of the indicated genotype were imaged for secreted mScarlet (ssmScarlet) and mStayGold::histone H2B fusions (mSG::H2B). A merged image overlaid on a DIC image is provided and the outline of the coelomocyte cell body is indicated by a dashed yellow line in the DIC overlap images. Images are representative of 20 young adult animals imaged over two independent experiments. We selected images where coelomocyte and muscle nuclei could both be imaged in a single DIC plane and animal anterior is oriented to the left. The top row contains images of the posterior pair of coelomocytes closest to the tail. The bottom three rows contain images of the mid-body pair of coelomocytes. Scale bars=10 µm.