Figure 2.

Workspace setup and serial filtration
(A) Tubes kept cold on a chilled MACS Chill 15 Rack during filtration.
(B) Filtration through a SmartStrainer into a 15 mL tube. Left (larger pore size): the pipette tip is held just above the mesh, without touching it. Right (30 μm): the pipette tip is in gentle contact with the mesh. Related to steps 6, 7, and 11.