Fig. 11.

VB-85387 reduces MASLD and fibrosis in HFD-fed STAM mice. Male STAM mice were generated as described in supplemental Fig. S2. Mice were fed an HFD for 16 weeks and treated with VB-85387 (30 mg/kg) or cpmparator (30 mg/kg) (n = 8). Quantitative histological analysis of stained liver sections was performed using Reveal Bioscience’s algorithm software. A: H&E and trichrome C staining of liver tissue sections. B: Percentage lipid area. C: Percentage fibrosis area. Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test, with comparisons made to chow-fed mice. Data are presented as mean ± SD. ∗∗P ≤ 0.001; ∗∗∗∗P ≤ 0.00001. MASLD, metabolic dysfunction-associated steatotic liver disease; HFD, high-fat diet; STAM, stelic animal model; H&E, hematoxylin and eosin.