Fig. 13.

VB-85387 suppresses IL-4/IL-6-mediated JAK1 activation. Liver samples were analyzed by ELISA or western blotting, as indicated (n = 8). A schematic of IL-4/IL-6-mediated JAK1 activation is depicted. A: IL-4 protein levels measured by ELISA. B: IL-6 protein levels measured by ELISA. C: pJAK1^{Tyr1022/Tyr1023} and total JAK1 protein levels determined by Western blot analysis. D: Relative pJAK1^{Tyr1022/Tyr1023} levels normalized to total JAK1 using ELISA (n = 8). Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test. Data are presented as mean ± SD. ∗∗∗∗P ≤ 0.00001. IL-4, interleukin-4; IL-6, interleukin-6; JAK, Janus kinase.