Fig. 3.

VB-85387 restores normal triglyceride levels in mice fed the LMCD diet. Male and female C57BL/6 mice were fed an LMCD diet as described in supplemental Fig. S1 (n = 8). A: A lipid tolerance test was performed on female mice. Mice were fasted and then administered olive oil by oral gavage. Blood triglyceride levels were measured at the indicated time points. B: AUC calculated from the results in panel A. C: liver triglyceride levels in male mice. D: liver triglyceride levels in female mice. E: Percentage fibrosis area in male mice. F: Percentage fibrosis area in female mice. Bioscience’s algorithm software was used to determine the percentage fibrosis area, with data calculated based on equal surface areas for all slides (57). Statistical analysis: two-way ANOVA with Tukey's post hoc test. Data are mean ± SD. ∗P ≤ 0.01; ∗∗P ≤ 0.001; ∗∗∗P ≤ 0.0001; ∗∗∗∗P ≤ 0.00001. LMCD, low methionine, choline-deficient; AUC, area under the curve.