Fig. 5.

VB-85387 reduces MASLD and fibrosis in LMCD-fed mice. Liver samples from each cohort were subjected to histological evaluation (n = 8). Tissue sections were stained with H&E or Masson’s trichrome C. Quantitative analysis of stained sections was performed using Reveal Bioscience’s algorithm software (57), with data calculated based on equal surface areas for all slides. A: H&E and Masson’s trichrome C staining of liver tissue sections. B: Percentage lipid area. C: Percentage fibrosis area. D: Immune cell density. Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test. Data are presented as mean ± SD. ∗∗P ≤ 0.001; ∗∗∗P ≤ 0.0001; ∗∗∗∗P ≤ 0.00001. MASLD, metabolic dysfunction-associated steatotic liver disease; LMCD, low methionine, choline-deficient; H&E, hematoxylin and eosin.