Fig. 7.

VB-85387 reduces type I and III collagen protein levels in mice fed the LMCD diet. Liver protein lysates were resolved on 4%–20% SDS-PAGE gels, transferred to nitrocellulose membranes, and probed with the appropriate antibodies. A: Western blot analysis of COL1A1 and COL3A1 protein levels in liver lysates, with GAPDH used as the loading control (n = 3).. The Western blot panels were generated from separate gels. For each gel, the protein of interest was blotted first; the membrane was then stripped and reprobed for GAPDH. B: COL1A1 levels determined by ELISA. C: COL3A1 protein levels determined by ELISA. D: αSMA levels determined by ELISA. Statistical analysis was performed using two-way ANOVA followed by Tukey’s post hoc test. Data are presented as mean ± SD. ∗∗∗P ≤ 0.0001; ∗∗∗∗P ≤ 0.00001. LMCD, low methionine, choline-deficient.