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VB-85387 increases Pparα signaling and inhibits Srebp signaling. Total RNA was collected from liver tissues and used for determining gene expression levels by qRT-PCR (n = 8). A: Srebp1-dependent gene expression levels of Srebf1, Acc1, and Fasn. B: Pparα-dependent gene expression levels of Pparα, Cpt1a, and Acot3. Statistical analysis: two-way ANOVA with Tukey's post hoc test. Data are mean ± SD. ∗∗∗P ≤ 0.0001; ∗∗∗P ≤ 0.0001.
