FIG. 2.

DC1 cells are superior in HIV-1 transmission. (A) Transmission assay as described in the legend of Fig. 1B. The amount of virus incubated with iDC or DC1 was titrated, and virus spread in SupT1 cells was measured. The DC1 cells were obtained by CD40L stimulation. (B) DC-mediated enhancement of HIV-1 infection. A minimal dose of 15 pg of CA-p24 per well of C33A-produced virus was used to infect T cells in the absence or presence of distinct DC subsets. No wash step was performed to remove unbound virus. (C and D) Transmission to primary T cells. DC were preincubated with virus (150 pg of CA-p24 per well), subsequently washed, and cocultured with PBL (C) or naïve T cells (Thn) (D). The CA-p24 values of day 4 posttransmission are shown.