FIG. 3.
(A) mDC subsets and stimulation of T cells. iDC were matured as described in the legend to Fig. 1. mDC (10 × 104 cells per well) were cultured in different numbers with 2.5 × 105 allogeneic naïve (CD45RA+) CD4+ T cells. After 5 days, T-cell proliferation was measured by [3H]thymidine incorporation (shown in cpm). Similar results were obtained with DC from two other donors. (B) HIV-1 transmission in mixed DC cultures. Transmission was performed with iDC, DC2, and a mixture of iDC and DC2. The CA-p24 values measured at day 4 posttransmission are shown.