FIG. 6.
Proliferation of HIV-1-specific CTL clones following stimulation with HIV-1-infected CD4+ T cells. CD4+ T cells infected with NL-432 or NL-M20A were cocultured with or without CTL clone SF2-33-135. Infected CD4+ T cells (105) were mixed with the same number of CTLs. On days 5, 10, and 15 postinfection, the number of total cells in each coculture was counted and the percentage of each type of cells in the coculture was determined by flow cytometry. The number of each type of cell in the coculture was calculated as follows: total cell number × percentage of each population.