Fig. 5. Time-lapse confocal imaging of selected mNG-2-NBAK Variants in Live E. coli. (A) Schematic of the time-lapse confocal imaging assay. (B) Representative time-lapse fluorescence images of E. coli expressing the WT mNG and the two selected variants at 25-frame intervals. (C) Photobleaching kinetics of the corresponding variants in live E. coli under continuous confocal laser scanning. Schematic diagrams were created with BioRender.com. mNG, mNeonGreen; WT, wild type.
