TABLE 4.

RBV induces mutations in replicating HCV RNA in HepG2 cells

Region and condition	Total no. of clones	No. (%) of mutant clones	Total no. of mutations	Nonsynonymous/synonymous substitutions (ratio)	Error generation rate (10−3)
Core
T7/H77	14	0	0	0	0
+ RBV (50 μM)	11	4 (36)	9	7/2 (3.50)	1.8a
E1/E2
T7/H77	12	2 (17)	2	1/1 (1.00)	0.33
+ RBV (50 μM)	14	7 (50)	15	10/5 (2.00)	2.1b
NS5B
T7/H77	10	0	0	0	0
+ RBV (50 μM)	12	3 (25)	4	2/2 (1.00)	0.79c
All regions
T7/H77	36	2 (5.5)	2	1/1 (1.00)	0.1
+ RBV (50 μM)	37	14 (38)	28d	19/9 (2.11)	1.6

^aOne nucleotide deletion (one stop codon). P < 0.001.

^bP < 0.01.

^cTwo nucleotide deletions (one stop codon).

^dP < 0.0001 relative to untreated T7/H77 by the χ² test with Yates' correction.