TABLE 1.
Intrahepatic Ag-specific IFN-γ+ cells after immunization
| Mouse group | Immunizing agenta | No. of IHLsb | % of CD8+ IHLsb | No. of CD8+ IHLsb | % of IFN-γ+/ CD8+b | No. of IFN-γ+ cells in the liver | Minimum no. of HBV-specific IHLs needed to exert effector functionc |
|---|---|---|---|---|---|---|---|
| 1 | ENV DNA/Vac | 2.2 × 106 | 48 | 1.1 × 106 | 0.09 | 9.5 × 102 | 3.0 × 104 |
| 2 | ENV DNA/DNA | 1.3 × 106 | 7.8 | 1.0 × 105 | 0.02 | 2.0 × 101 | 3.0 × 104 |
| 3 | POL DNA/Vac | 2.5 × 106 | 46 | 1.2 × 106 | 7.3 | 8.4 × 104 | 4.5 × 105 |
| 4 | POL DNA/DNA | 2.0 × 106 | 8.2 | 1.6 × 105 | 2.7 | 4.4 × 103 | 4.5 × 105 |
a
HBV transgenic mice were immunized by DNA and administered booster injections of vaccinia virus (Vac) or DNA.
b
IHLs were harvested 10 days after the second immunization, and data represent the averages of results for three or four mice per group.
c
Data from results presented in Table 2.