FIG. 5.

Cell-free nuclear import assays. (A) Uninfected cells (mock infected) or cells that had been infected with rhinovirus for 6 h were permeabilized and used in an in vitro nuclear import assay. Assays were carried out in the presence (+RRL) or absence (−RRL) of RRL as a source of cytosolic factors. Top panels show GFP with an FITC filter, and bottom panels show Hoechst staining of DNA with a UV filter. All GFP images were acquired in Photoshop 5.0 (Adobe Systems, Inc.) with identical exposure times and manipulations. (B) Same as panel A, except creatine kinase, creatine phosphate, ATP, and GTP were omitted from the reaction. GFP images were acquired with the same exposure time as panel A.