TABLE 1.
Profiles of HIV-1-infected patients and virus isolated from their blood
| Patient | Clinical statusa | CD4 count (cells/μl) | Plasma HIV-1 RNA (copies/ml)b | Plasma HIV-1 HMA resultc | T-cell-associated virus
|
B-cell-associated virus
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HIV-1 RNA (copies/106 cells) | Days in cultured | p24, pg/mle | Coreceptor usagef | HIV-1 RNA (copies/106 cells) | Days in cultured | p24, pg/mle | Coreceptor usagef | |||||
| 1 | AIDS, nonadherence | 202 | 11,232 | Low | 26,000 | 3 | 365 (4) | R5 | 4,600 | 3 | 100 (3) | R5 |
| 2 | Asym, naive | 483 | 100,730 | High | 15,000 | 3-4 | 320 (3) | R5 | 4,700 | 3-4 | 120 (2) | R5 |
| 3 | AIDS, nonadherence | 987 | 62,743 | High | 35,000 | 3 | 100 (4) | R5 | 17,000 | 3 | 100 (3) | R5 |
| 4 | AIDS, nonadherence | 188 | 249,966 | Mod | NDg | 3 | 400 (4) | R5 | ND | 5 | 1,700 (7) | R5 |
| 5 | AIDS, nonadherence | 6 | 417,389 | High | 65,000 | 3-7 | 135 (5) | X4 (R5) | 6,500 | 4 | 115 (4) | X4 (R5) |
| 6 | AIDS, nonadherence | 92 | 16,989 | High | 5,400 | 2 | 4,000 (2) | X4 (R5) | 1,400 | 2-4 | 750 (2) | X4 (R5) |
| 7 | AIDS, failure | 108 | 133,822 | Low | 44,000 | 3 | 100 (3) | R5 | 17,000 | 3 | 100 (2) | R5 |
| 8 | AIDS, nonadherence | 136 | 47,489 | High | 12,000 | 3 | 200 (3) | X4 (R5) | 1,000 | 3 | 110 (3) | X4 (R5) |
| 9 | AIDS, nonadherence | 170 | 65,433 | Mod | 55,000 | 3 | 1,400 (4) | X4 | 13,000 | 3 | 100 (3) | X4 |
| 10 | AIDS, failure | 6 | 194,638 | Low | 6,700 | 4 | 188 (7) | X4, R5 | 8,300 | 4 | 253 (6) | X4, R5 |
| 11 | AIDS, failure | 53 | 244,174 | High | 51,000 | 3 | 200 (4) | X4 | 4,700 | 3 | 700 (2) | X4 |
| 12 | Asym, interruption | 316 | 166,143 | Mod | 42,000 | 4 | 1,316 (1) | R5 | 14,000 | 4 | 110 (1) | R5 |
| 13 | Asym, interruption | 1,573 | 109,850 | High | 1,800 | 4 | 250 (1) | R5 | 1,000 | 4 | 284 (1) | R5 |
| 14 | Asym, interruption | 797 | 241,785 | Low | ND | 3 | 100 (4) | R5 | ND | 6 | 250 (1) | R5 |
| 15 | Asym, interruption | 200 | 116,083 | Low | 19,000 | 3 | 760 (7) | R5 | 600 | 9 | 1,100 (2) | R5 |
Asym, aymptomatic; nonadherence, naive, failure, and interruption refer to antiretroviral therapy.
As measured by the Chiron bDNA assay, with a detection limit of 50 copies/ml.
Level of heterogeneity observed in HIV-1 RNA derived from 0.1 ml of plasma; low, 1 to 3 distinct bands; mod, 1 to 3 distinct bands with smears or 4 to 6 distinct bands; high, >6 bands with or without smears. Mod, moderate.
Coculture day on which supernatants were collected for HIV-1 RNA isolation.
Average release of p24 in supernatants of pooled wells (number of wells pooled).
Coreceptor usage of isolated virus was evaluated with U87-CD4 cell lines expressing CCR5 (R5) or CXCR4 (X4). R5 in parentheses indicates minor usage of CCR5.
ND, not determined.