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. 2002 Sep;76(17):8582–8595. doi: 10.1128/JVI.76.17.8582-8595.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 10. — Analysis of the 76-kDa proteinase-polymerase precursor (Pro-Pol) in RC pellets collected over time from FCV-infected cells. The presence of Pro-Pol in RCs collected over the 8-h time course was analyzed with two different antisera. (A) Mock-infected (M) RCs (lane 3) or FCV-infected RCs collected over an 8-h time course (lanes 4 to 11) were probed with guinea pig hyperimmune sera prepared against the N terminus (amino acids 1072 to 1192) of the recombinant proteinase (anti-rPro). The rPro-Pol protein (lane 2) and FCV-infected TL collected 8 h postinfection (lane 12) were included as controls. (B) Mock-infected (M) RCs (lane 3) or FCV-infected RCs collected over an 8-h time course (lanes 4 to 11) were probed with guinea pig hyperimmune sera prepared against the rPro-Pol protein (anti-rPro-Pol). The rPro-Pol protein (lane 2) and FCV-infected TL collected at 8 h postinfection (lane 12) were included as controls. Lane 1 in each panel contains See Blue Plus protein molecular size markers (Invitrogen). The values on the left are molecular sizes in kilodaltons.