FIG. 5.

Time course analysis of RNA synthesis in an individual replication assay. A replication assay was initiated in the presence or absence of [α-³²P]CTP, and aliquots were taken at the indicated time points. (A) Analysis of radiolabeled RNA collected at 0, 1, 10, 30, and 60 min (lanes 1, 2, 3, 4, and 5, respectively). (B) Northern blot analysis of nonradiolabeled RNA samples collected at 0, 1, 10, 30, and 60 min (lanes 1, 2, 3, 4, and 5, respectively) and probed with an antisense RNA probe that detects positive-sense RNA synthesis. Lane 6 contains full-length positive-sense RNA transcripts (designated + RNA) derived from linearized pQ14. (C) Northern blot analysis of nonradiolabeled RNA samples collected at 0, 1, 10, 30, and 60 min (lanes 1, 2, 3, 4, and 5, respectively) and probed with a sense RNA probe that detects negative-sense RNA synthesis. Lane 6 contains full-length RNA transcripts (positive sense) (designated + RNA) derived from linearized pQ14.