FIG. 6.

Analysis of RNA synthesis from RC pellets collected over time from FCV-infected cells. Cells were infected with FCV at an MOI of 10 and, beginning at 1 h postinfection (PI), collected hourly for isolation of RCs. The RCs isolated at each time point were analyzed for the ability to synthesize RNA in an in vitro replication assay. (A) Analysis of radiolabeled RNA synthesized from RC pellets collected hourly for up to 8 h after infection (lanes 2 to 9). (B) Northern blot analysis of nonradiolabeled RNA synthesized from RC pellets collected at 1 to 8 h postinfection (lanes 2 to 9) probed with an antisense RNA probe that detects positive-strand RNA synthesis. (C) Northern blot analysis of nonradiolabeled RNA synthesized from RC pellets collected at 1 to 8 h postinfection (lanes 2 to 9) probed with a sense RNA probe that detects negative-strand RNA synthesis. Lane 1 contains RNA purified from a replication assay performed under the same conditions with a pellet isolated from mock-infected cells (M).