FIG. 1.

CB3-RD infects polarized T84 cells more efficiently than does CB3-Nancy. Polarized T84 cells (A) or CHO cells expressing CAR (B) were exposed to CB3-RD and CB3-Nancy (10 PFU/cell), washed, and then incubated at 37°C. Cells were fixed and stained with an anti-CB3 mouse monoclonal antibody followed by a FITC-conjugated secondary antibody. Cells were visualized by immunofluorescence microscopy, and viral antigen-positive cells were quantified by examining digital photographs of random fields. Graphs show the average number of antigen-positive cells per field and standard deviations (error bars) for three fields. Results are representative of three experiments.