FIG. 5.

Anti-DAF antibody inhibits the infection of polarized T84 cells by CB3-RD. (A) Polarized T84 monolayers were preincubated with control monoclonal antibody, anti-CAR monoclonal antibody, or anti-DAF antibody before infection by CB3-RD. Infected cells were visualized by staining for CB3 viral antigen with a polyclonal horse anti-CB3 serum followed by a secondary antibody conjugated to FITC and examination by immunofluorescence microscopy. The graph shows the average number of viral antigen-positive cells per field (± standard deviation) for three fields. Results are representative of four experiments. (B) Control CHO-CAR cells were preincubated with a control antibody, an anti-CAR antibody, or an anti-DAF antibody before exposure to CB3-RD. Cells were stained for CB3 viral antigen.