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Passage of CB3-Nancy on T84 monolayers selects for DAF-binding variants. CB3-Nancy was passaged on T84 monolayers as described in Materials and Methods. After the second (A) and fourth (B) serial passages, viruses were radiolabeled and binding to transfected CHO cell monolayers was measured. The graph shows mean cpm bound (± standard deviation) for triplicate samples.
