TABLE 2.
Chemokine release from influenza A virus-infected cellsa
| Cells | Stimulus | Chemokine concn (ng/ml)
|
|||
|---|---|---|---|---|---|
| CXCL1 (GRO-α) | CCL5 (RANTES) | CCL3 (MIP-1α) | CCL2 (MCP-1) | ||
| NHBE | Control | ND | ND | ND | ND |
| A/PR/8/34 | ND | ND | ND | ND | |
| U1752 | Control | 0.03 ± 0.02 | 0.07 ± 0.01 | ND | 0.09 ± 0.01 |
| A/PR/8/34 | 0.50 ± 0.03 | 0.29 ± 0.01 | ND | 0.09 ± 0.02 | |
| A549 | Control | 0.70 ± 0.07 | 0.07 ± 0.01 | ND | 1.60 ± 0.14 |
| A/PR/8/34 | 5.00 ± 0.07 | 8.20 ± 0.40 | ND | 4.92 ± 0.41 | |
a
NHBE, U1752, and A549 cells (5 × 105/ml) were incubated with or without A/PR/8/34 (MOI, 2), and after 48 h the cell culture supernatants were harvested and the content of chemokines was determined by specific ELISAs. Values represent the mean ± standard deviations of triplicate incubations. ND, not detectable.