FIG. 5.

Inhibition of HIV-1-induced neuronal apoptosis by NMDA receptor antagonists. Neuronal cultures were preincubated with various antagonists for 90 min prior to exposure to macrophage supernatants and examined by TUNEL assay 48 h later as described in Fig. 3. Solid bars represent cultures exposed to HIV/MDM medium (Mφ/89.6) or mock-infected (Mφ uninfect) medium. Hatched bars represent control cultures exposed to antagonist or antibody alone. (A) Individual cultures were preincubated with antagonists as follows: MK801 (10 μM), D-AP5 (1 mM), l-NAME (100 μM), or CNQX (30 μM). The data represent one of three independent replicate experiments. (B) Individual cultures were preincubated for 60 min with MK801 (10 μM), anti-CXCR4 MAb (12G5; 10 μg/ml), anti-CCR5 MAb (10 μg/ml), IgG2A isotype control antibody, or IgG2B isotype control antibody. The data represent average of two to three independent experiments. Results were expressed as mean ± standard error.