FIG. 7.

Resistance of NT2.N/bcl-2 and NT2.N/bcl-xL neurons to HIV-1-induced apoptosis. NT2.N neurons derived from stably transfected NT2 cell lines were cultured on a feeder layer of astrocytes for 5 weeks, exposed to HIV-1/MDM culture supernatants, and then assayed by TUNEL for apoptotic neurons as described in the legend to Fig. 3. Results are shown as the relative percentage of neuronal apoptosis in NT2.N, NT2.N/bcl-2, and NT2.N/bcl-xL neurons similarly exposed to supernatants derived from MDM independently infected with three different HIV-1 isolates. Results are expressed as the mean percentage of TUNEL-positive neurons from two independent experiments ± standard error.