FIG. 4.

Effect of ncp BVDV infection on induction of MxA by dsRNA or by IFN-α in CaTe cells. CaTe cells were either mock infected (a and c) or infected at a high MOI with ncp BVDV (b and d) for 48 h. Cells were then treated for 20 h with fresh medium containing either various concentrations of recombinant bovine IFN-α1 (a and b) or the synthetic dsRNA poly(I)·poly(C) (c and d) at increasing concentrations as described in Materials and Methods. Cells were harvested in sample buffer and subjected to polyacrylamide gel electrophoresis and immunoblotting using a rabbit antiserum against human MxA. Lanes marked + in panels a and b indicate treatment with 10 μg of poly(I)·poly(C)/ml. M, mock treated. MxA (a, b, and c) or its expected position (d) is shown.