FIG. 5.

ncp BVDV inhibition of IFN-β gene transcription. CaTe cells were first either mock infected or infected with ncp BVDV (1 to 2 PFU/cell) for 48 h and then either mock infected or infected with SFV (5 PFU/cell) for the times indicated, or treated with poly(I)·poly(C) (dsRNA) by addition to the medium at 100 μg/ml for 2 h. Total RNA was prepared from the cells and analyzed for the presence of IFN-β and γ-actin mRNAs by RNase protection. The actin transcript is indicated. Multiple IFN-β transcripts are detected due to the limited cross-homology to the bovine probe used and the existence of at least three bovine IFN-β genes.