TABLE 1.
Effect of ncp BVDV on IFN-α/β induction by SFV
| Virus or cell treatmenta | Mean IFN-α/β titer (U/ml) | Range of titer (U/ml) |
|---|---|---|
| Mock infected CaTe cells | 0.0 | 0.0 |
| ncp BVDV (24 h) | 0.0 | 0.0 |
| ncp BVDV-SFV (24 h) | 1.8 | 0.8-3 |
| SFV (24 h) | 177 | 65-289 |
| ncp BVDV (48 h) | 0.0 | 0.0 |
| ncp BVDV-SFV (48 h) | 0.35 | 0.0-0.7 |
| SFV (48 h) | >300 | >300 |
a
CaTe cells in 6-well plates were either mock infected or infected with ncp BVDV at 1 to 2 PFU/cell and incubated for 2 days. Duplicate cultures were subsequently infected with 100 PFU of SFV, and samples of medium were taken at 24 and 48 h following infection with SFV. Following virus inactivation, samples were analyzed for IFN-α/β production by a bovine IFN-α/β Mx-CAT reporter assay (14).