FIG. 1.
NSP1 interacts with IRF-3. Bovine NSP1 (bNSP1) and murine NSP1 (mNSP1) in the yeast two-hybrid bait vector were tested for interaction with IRF-3 in the activation domain vector. Schematic diagrams indicate the quadrants of selection media where yeast containing the indicated plasmids were streaked. (A) Interaction between bovine NSP1 and IRF-3, or between murine NSP1 and IRF-3, on nutrient selection medium; (B) interaction of bovine NSP1 with simian virus 40 T antigen in the activation domain plasmid (left panel), and IRF-3 interaction with an irrelevant protein (v15) in the DNA binding domain plasmid (right panel). YPAD, yeast extract-peptone-adenine-dextrose.