FIG. 2.
NSP1 interacts with IRF-3 in a GST pull-down assay. IRF-3 was expressed in bacteria as a GST fusion protein. GST-IRF-3 or GST alone was bound to glutathione-Sepharose 4B, and radiolabeled mock-infected (M) or B641-infected (I) lysates were incubated with the beads. (Left panel) The arrow indicates an ∼50-kDa NSP1 pulled down by GST-IRF-3. Asterisks indicate VP7 (∼38 kDa) and NSP3 (∼34 kDa). M and I lanes contain lysates not subjected to the pull-down assay. (Right panel) Western blot of GST-IRF-3 eluates with an anti-NSP1 monoclonal antibody (provided by R. L’Haridon and D. Poncet, INRA, Jouy-en-Josas, France). Molecular mass markers are noted at the left.