TABLE 2.

Outcome for fetal rhesus monkeys (M. mulatta) intracranially inoculated with RhCMV-EGFP in utero at day 50 of gestation^a

Fetus (i.d. no.)	Inoculum titer (PFU)	Necropsy (dpi)	Sonographic findings	Gross findings at necropsy	Viral IE1 antigen found by IHC	Virus isolation
						10 dpi	22 ± 1 dpi
1 (001-0645)	5 × 104	NDb	Microcephalyc	NDb	NDb	ND	ND
2 (001-0801)	1 × 105	10	Flocculent AF	Mild ventricular dilatation	Brain	ND	ND
			Ventricular dilatation	Lower limb deformity
			Severe hydrops	Ileocolic stricture, dilated colon
			Microcephaly
			Echogenic bowel
3 (001-0879)	1 × 105	21	Severe hydrops	Ventricular dilatation	Brain	−(FB)	+(FB)
			Severe microcephaly	Lower limb deformity	Adrenals	−(AF)	−(P)
			Abdominal distension	Ileocolic stricture, dilated colon	Kidney		−(AF)
			Lower limb deformity	SD (cranium and neck)	Liver
			IUGR		Gonad
					Pancreas
4 (001-0888)	1 × 105	23	Severe microcephaly	Ventricular dilatation	Brain	+(FB)	+(FB)
			Severe hydrops	Lower limb deformity	Adrenal	−(AF)	+(P)
			Abdominal distension	Ileocolic stricture, dilated colon	Kidney		−(AF)
			Lower limb deformity	SD (cranium, face, and neck)	Liver
			IUGR		Gonad
					Pancreas
					Lung
					Duodenum
					Colon

^ai.d., identification; ND, not done; AF, amniotic fluid; IUGR, intrauterine growth restriction; SD, subcutaneous discoloration; IHC, immunohistochemistry; FB, fetal blood; P, plasma.

^bFetus death at ≈22 dpi, which was attributed to virus pathogenicity.

^cEvident within 10 dpi, severe at death.