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. Author manuscript; available in PMC: 2006 Feb 13.
Published in final edited form as: Hum Gene Ther. 2005 Feb;16(2):235–247. doi: 10.1089/hum.2005.16.235

FIG. 3.

FIG. 3

Transduction of rat aortic endothelial cells (RAEC) with recombinant adeno-associated virus (rAAV) serotypes. RAEC were cultured to approximately 50% confluence in 24-well plates and transduced with rAAV serotype vectors containing human α1-antitrypsin (hAAT) at a multiplicity of infection (MOI) of 1000 viral particles per cell. No adenovirus (Ad) coinfection was used in these experiments. Media were collected at days 7, 14, and 21 after transduction and hAAT levels were measured by enzyme-linked immunosorbent assay (ELISA) as described in Materials and Methods. Values are expressed as mean ± standard error of the mean (SEM), n = 4; *represents p < 0.05 vs. rAAV2, 3, 4, and 5 (at days 7 and 21); #represents p < 0.05 vs. rAAV2, 3, and 4.