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. Author manuscript; available in PMC: 2006 Feb 13.
Published in final edited form as: J Biol Chem. 2000 Jan 14;275(2):1247–1260. doi: 10.1074/jbc.275.2.1247

Fig. 9.

Fig. 9

Inhibition of GRK5 activity by CaBP1. Purified GRK5 (0.8 pmol) were used to phosphorylate rod outer segment membranes (2 μm rhodopsin) in the presence of indicated concentrations of CaBPs. Proteins were separated on a 10% SDS-polyacrylamide gel and visualized by autoradiography. 32P incorporation into proteins was determined by excising and counting the radioactive bands. The activity of GRK5 in the presence of CaBPs is expressed as a percentage of the rhodopsin phosphorylation in the absence of CaBPs. The short forms CaBP1 and CaBP2 were used in the assays.