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. Author manuscript; available in PMC: 2006 Feb 13.
Published in final edited form as: J Immunol. 2005 Nov 1;175(9):5708–5715. doi: 10.4049/jimmunol.175.9.5708

FIGURE 7.

FIGURE 7.

STAT6 is required for IL-4 up-regulation of Ugrp2 gene transcription. A, The presence of STAT6 in wild-type, but not STAT6-deficient (STAT6-/-), mouse embryonic lung primary culture cells. Expression of STAT6 was detected by Western blotting using a rabbit polyclonal anti-STAT6 Ab. B, Expression of IL-4Rα and IL-13Rα1 and absence of γc in lung primary culture cells. RT-PCR was conducted using RNAs prepared from embryonic lung primary culture cells of wild-type and STAT6-deficient (STAT6-/-) mice for IL-4Rα and IL-13Rα1, and from wild-type mice for γc. Spleen was used as a positive control for γc. The sizes of PCR products are 432 bp (IL-4Rα), 385 bp (IL-13Rα1), 191 bp (18S), 266 bp (γc), and 452 bp (GAPDH). C, STAT6 is activated by IL-4 in lung primary culture cells. Wild-type lung primary culture cells were treated with 50 ng/ml IL-4 for 30 min. The level of activated STAT6 was measured by Western blotting using a rabbit polyclonal anti-phospho-STAT6 (Tyr641) Ab. D, Northern blot analysis of total RNAs (20 μg/lane) prepared from wild-type or STAT6-/- mouse embryo lung primary culture cells treated with IL-4 (0, 1, 10, and 100 ng/ml) for 24 h. Hybridization was serially conducted using a probe for Ugrp2 and 18S.