FIG. 4.

HUVEC growth adaptation of RV-FIX (A, C, E, and G) and RVΔUL45 (B, D, F, and H) after passage 3 (A and B), passage 5 (C and D), and passage 15 (E to H) performed according to a reported protocol (7). Medium-sized plaques in panels A and B became larger plaques in panels C and D until the spreading of viral infection to the entire cell monolayer in panels E through H. (A to F) Indirect immunofluorescence staining with a combination of monoclonal antibodies to p72 (nuclear staining) and gB (cytoplasmic staining). The gB monoclonal antibody was kindly provided by Lenore Pereira (University of California, San Francisco). (G and H) Cytopathic effect of infected HUVEC as observed in living cells at passage 15.